Not really, especially if you're a foreigner it sounds more like you're mixing your genders than doing it on purpose.

There are better ways to insult masculinity/femininity that clearly state your purpose.

Honestly, not really. They might think you're just confused. Misgendering in that way doesn't come across as offensive in Spanish as it does in English. There are way more effective ways to insult someone, even if you're still aiming to demasculate them.