2SLGBTQ+ is used mostly by the CBC, since it's the most Canadian version, highlighting two-spirit as something culturally distinct. But in practice, we all just use "LGBT+ or "LGBTQ".

Kinda like how we all use "MMIWG" and not "MMIWGA2SLGBTQI+P" https://mmiwg2splus-nationalactionplan.ca/eng/1670511213459/1670511226843

(Also to be pedantic, it's an initialism)