You can do that with B2. Just use an application to upload that encrypts as it uploads.
The only way to achieve the same on the backup plan (because you have to use their desktop app) is to always have your entire system encrypted and never decrypt anything while the desktop app is performing a backup.
Did you not read what I said? You use their app, which copies files from your system as-is. Ensuring it never grabs a cleartext file is not practical.