Sure, but I think most people would look at that moment and find it to be a touching gesture. There are exceptions where even macho men accept emotional moments. Stuff like this and war related stuff is what, in my experience, many men shed secret tears to.
There's a reason that for example, Band of Brothers is still regarded as one of the best TV series about war ever. There are plenty of men crying in that show and every fan community I have visited, men are getting very emotional about that show and things like it.
I think we are doing all men a disservice if we just assume that the majority of them never allow themselves or other men to show emotions, because that is not true. It just tends to happen in a bit of a different way than the way women express emotions.
And their definitely are people out there who would look at that moment with the astronauts and go "GAAAYYY" but I don't think they are in the majority at all.
In my experience both on and offline, men get emotional and show a lot of empathy for situations like this one. Maybe they don't cry themselves, but they respect another man honoring his wife's memory and will defend his right to that moment.