▲ 8 ▼ 300x - Darkfield Algae (video.nyc) submitted 10 months ago by cybervseas@lemmy.world to c/microscopy@mander.xyz 4 comments fedilink hide all child comments My green water culture is challenging to study with the microscope but still a lot of fun.
[–] pebbles@sh.itjust.works 2 points 10 months ago (3 children) Looks great! I feel the pain on trying to get higher magnification darkfield to work lol. permalink fedilink source hideshow 3 child comments replies: [–] cybervseas@lemmy.world [S] 2 points 10 months ago (2 children) I think I need to get my objective and condenser aligned better to get this to work at higher magnifications. I wish I understood how to do that. Right now otherwise at 40x objective only the specimen near the top of the fov will actually be visible. And also I get a gradient on the background instead of solid dark field. permalink fedilink source parent hideshow 2 child comments replies: [–] pebbles@sh.itjust.works 2 points 10 months ago (1 child) Ah I didn't realize that aligning the condenser could help. I'm going to try that. I tend to get gradients in my background on higher magnifications as well, but since I do more artsy things I tend to use that to my advantage with rhienburg illumination mixed in. It can look quite nice. permalink fedilink source parent hideshow 1 child comment replies: [–] cybervseas@lemmy.world [S] 2 points 10 months ago Look i say this, but remember i barely know anything here. Still not sure even sure how all those parts are connected. But for setting up Kohler illumination it looks to be essential. Yesterday I properly aligned the light source on my microscope and wow it was a tremendous improvement in available light. Feels at least 50% brighter. https://evidentscientific.com/en/microscope-resource/knowledge-hub/techniques/darkfieldsetup permalink fedilink source parent
[–] cybervseas@lemmy.world [S] 2 points 10 months ago (2 children) I think I need to get my objective and condenser aligned better to get this to work at higher magnifications. I wish I understood how to do that. Right now otherwise at 40x objective only the specimen near the top of the fov will actually be visible. And also I get a gradient on the background instead of solid dark field. permalink fedilink source parent hideshow 2 child comments replies: [–] pebbles@sh.itjust.works 2 points 10 months ago (1 child) Ah I didn't realize that aligning the condenser could help. I'm going to try that. I tend to get gradients in my background on higher magnifications as well, but since I do more artsy things I tend to use that to my advantage with rhienburg illumination mixed in. It can look quite nice. permalink fedilink source parent hideshow 1 child comment replies: [–] cybervseas@lemmy.world [S] 2 points 10 months ago Look i say this, but remember i barely know anything here. Still not sure even sure how all those parts are connected. But for setting up Kohler illumination it looks to be essential. Yesterday I properly aligned the light source on my microscope and wow it was a tremendous improvement in available light. Feels at least 50% brighter. https://evidentscientific.com/en/microscope-resource/knowledge-hub/techniques/darkfieldsetup permalink fedilink source parent
[–] pebbles@sh.itjust.works 2 points 10 months ago (1 child) Ah I didn't realize that aligning the condenser could help. I'm going to try that. I tend to get gradients in my background on higher magnifications as well, but since I do more artsy things I tend to use that to my advantage with rhienburg illumination mixed in. It can look quite nice. permalink fedilink source parent hideshow 1 child comment replies: [–] cybervseas@lemmy.world [S] 2 points 10 months ago Look i say this, but remember i barely know anything here. Still not sure even sure how all those parts are connected. But for setting up Kohler illumination it looks to be essential. Yesterday I properly aligned the light source on my microscope and wow it was a tremendous improvement in available light. Feels at least 50% brighter. https://evidentscientific.com/en/microscope-resource/knowledge-hub/techniques/darkfieldsetup permalink fedilink source parent
[–] cybervseas@lemmy.world [S] 2 points 10 months ago Look i say this, but remember i barely know anything here. Still not sure even sure how all those parts are connected. But for setting up Kohler illumination it looks to be essential. Yesterday I properly aligned the light source on my microscope and wow it was a tremendous improvement in available light. Feels at least 50% brighter. https://evidentscientific.com/en/microscope-resource/knowledge-hub/techniques/darkfieldsetup permalink fedilink source parent
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